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An assay is a procedure in molecular biology for testing and/or measuring the activity of a drug or biochemical in an organism or organic sample. A quantitative assay may also measure the amount of a substance in a sample. Bioassays and immunoassays are among the many varieties of specialized biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding. Assay varietiesThis section does not cite any references or sources. Please help improve this article by adding citations to reliable sources. Unsourced material may be and removed. (May 2009)CytotoxicityA cytotoxicity assay measures how toxic a chemical compound is to cells.
DNAAssays for studying interactions of proteins with DNA include: From Wikipedia under the
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Trading Markets (press release) Assay results have been received for three of the holes; remaining assays are expected by the New Year. Drilling is expected to recommence in January 2010. ... and more » SMW Gold Announces Assay Results for Gold Deposits in Egypt
SYS-CON Media (press release) Mark Lisnyansky, Managing Director of SMW Gold, said, "Our latest assay report indicates compelling prospects for SMW Gold's exploration in El Fawakhir and ... and more » Picchu Rio Gold Mining Co. SAC announces initiation of river mining of their ...
Earthtimes (press release) (Business Wire: June 13, 2002) A March 1, 2002 Certificate of Assay on the holding's Rare Earths by a leading and independent analysis and testing firm, ... and more » From Google News Search: "Assay" What are the precautions taken to ensure accuracy in carrying out a colorimetric enzyme assay? Q. Besides keeping volume of solutions, timing, temperature constant. Thank you. Asked by sky_blue - Mon Sep 3 08:20:07 2007 - - 1 Answers - 0 Comments A. Besides volume of solutions, timing, and constant temperature, one important step that should be taken is to have a good standard curve. A good standard curve should show a mathematic relationship between the concentrations of enzyme used and the colorimetric readout. A good standard curve can be used as a good internal control for many differing variables. As absorption of light will be measured, the spectrophotometer should be calibrated, or rather, steps should be taken to make sure it is working as expected. A good standard curve can tell you if it is working properly. The experiment should contain 'zeros' in which no enzyme is added at all. You mentioned time as a variable which was already considered. This can be an especially… [cont.] Answered by cl3v3r boy - Mon Sep 3 09:55:19 2007 How does methylation interference assay works? Q. I'm reading a paper where they used this assay to determine which nucleotides participate in binding of a transcription factor. I, however, have not previously learned about this assay and don't seem to find a good description and explanation of its principles online. Can you explain in or direct me to a site where it is explained? Asked by Kaytee - Mon Feb 26 14:46:21 2007 - - 1 Answers - 0 Comments A. Answered by ger - Mon Mar 5 16:01:58 2007 Why do we need to dilute proteins for the Bradford Assay?
Q. What is the reason we dilute the proteins and not just use the original cell fractions? Do we need a solution that has more or less protein in it to get a good absorbance? Asked by ruz - Mon Jul 6 15:25:29 2009 - - 3 Answers - 0 Comments A. Beer's Law breaks down at high concentrations. Dilute samples give a reaction on the linear portion of the curve. One of the disadvantages of the assay is that it has only a fifty- to one-hundred-fold working range. Answered by novangelis - Mon Jul 6 15:35:37 2009 From Yahoo Answer Search: "Assay" |






