Metallic Screen assays confirm high grade gold results at Crowsnest

PR Newswire (press release)

V: MXR; OTCBB: MXROF; Frankfurt : M1D) has received the results of the Metallic Screen assays conducted on all samples from its recent reverse circulation ...



and more »

POPULARity contest: For platelet function assays , what works, what doesn't

TheHeart.Org

Orlando, FL - Results for the first head-to-head comparison of different platelet-function tests suggests that a handful of platelet reactivity assays used ...



and more »

Prodesse Gets FDA OK for Parainfluenza Virus Assay

GenomeWeb Daily News

The assay uses real-time RT-PCR to identify the parainfluenza 1, 2, and 3 viruses from nasal swabs. It joins a line of molecular assays for respiratory ...

How can the use of detergents in a protein assay effect the protein concentrations?
Q. Detergent solution used in this protein assay to make up samples containing a known concentration of BSA, aswell as detergent.
Asked by Issy - Sat Feb 28 19:49:42 2009 - - 1 Answers - 0 Comments

A. The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method to be used on detergent treated membrane preparations.
Answered by unknown - Sun Mar 1 07:24:07 2009

How do lipids, bilirubin, hemoglobin and ammonia interfere with biuret assay of total proteins?
Q. I can't find the answers in the internet. They just keep on saying that those interfere with the assay. But they don't specifically tell how each of those interfere with the assay. I need your help badly. Thanks!
Asked by hermione - Tue Sep 2 07:42:40 2008 - - 1 Answers - 0 Comments

A. Read that article
Answered by adel a - Sat Sep 6 07:55:18 2008

You are going to do an enzyme assay, and you need to make a solution of substrate.?
Q. The concentration of substrate that you will use is 0.001 ug/ml. It is necessary to have enough for the enzyme assay. You will need to test 50 samples, plus a negative control lacking the enzyme, and another negative control lacking the substrate. You have determined that you will do all of this in triplicate. The assay calls for 1 ml of substrate solution and 0.2 ml of enzyme solution. If you don't have enough substrate solution, you will have to make more in the middle of the experiment, which could ruin your results. a. How much substrate solution should you make? b. How many grams of substrate will you need for this solution? c. How can you make this solution if you don't have a balance accurate enough to weigh such a small quantity? I… [cont.]
Asked by Lena - Fri Feb 23 07:16:10 2007 - - 1 Answers - 0 Comments

A. a. How much substrate solution should you make? 50 samples + a negative control (do all of this in triplicate) The assay calls for 1 ml of substrate solution Therefore, (50 + 1)x1 ml x 3 = 153 ml b. How many grams of substrate will you need for this solution? concentration of substrate that you will use is 0.001 ug/ml Therefore, 153 ml x 0.001 ug/ml = 0.153 ug c. How can you make this solution if you don't have a balance accurate enough to weigh such a small quantity? Weigh 1.0mg of substrate and dissolve it in 1000 ml buffer solution in a volumetric flask (stock solution). The resulting concentration will be 1.0 mg/1000ml = 0.001mg/mL (i.e. 1 ug/ml ) Take 1ml from stock solution and dilute it to 1000 ml with buffer solution in a… [cont.]
Answered by Yy - Fri Feb 23 10:03:34 2007