An assay is a procedure in molecular biology for testing and/or measuring the activity of a drug or biochemical in an organism or organic sample. A quantitative assay may also measure the amount of a substance in a sample. Bioassays and immunoassays are among the many varieties of specialized biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding.

Assay varieties

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Cytotoxicity

A cytotoxicity assay measures how toxic a chemical compound is to cells.

DNA

Assays for studying interactions of proteins with DNA include:

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Mon Jan 25 03:13:34 2010

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Mon Jan 18 03:21:52 2010

MAG Silver announces gold and molybdenum assay results
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MAG Silver announces gold and molybdenum assay results

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MAG Silver Corp. (TSX:MAG)(NYSE Amex:MVG) reported additional gold and molybdenum . assay. results from.

REMIXX WORLD!: Duke Awarded Up to $43 Million By Biomedical ...
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REMIXX WORLD!: Duke Awarded Up to $43 Million By Biomedical ...

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We believe that using our diagnostic . assay. in such points would allow us to screen tens of thousands of individuals in a 24-hour period and to distinguish true radiation victims from the uninjured," says Chute. Chute says the . assay. will ...

Leveraging Assay Platforms to Create Category-Killer Diagnostic ...
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Leveraging . Assay. Platforms to Create Category-Killer​ Diagnostic Tests on IN VIVO, Dec-01-2009.

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Thu Jan 21 06:18:47 2010

Metallic Screen assays confirm high grade gold results at Crowsnest - PR Newswire (press release)
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Metallic Screen assays confirm high grade gold results at Crowsnest

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V: MXR; OTCBB: MXROF; Frankfurt : M1D) has received the results of the Metallic Screen assays conducted on all samples from its recent reverse circulation ...



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Prodesse Gets FDA OK for Parainfluenza Virus Assay - GenomeWeb Daily News
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Fri Dec 11 13:32:45 2009

How can the use of detergents in a protein assay effect the protein concentrations?
Q. Detergent solution used in this protein assay to make up samples containing a known concentration of BSA, aswell as detergent.
Asked by Issy - Sat Feb 28 19:49:42 2009 - - 1 Answers - 0 Comments

A. The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method to be used on detergent treated membrane preparations.
Answered by unknown - Sun Mar 1 07:24:07 2009

How do lipids, bilirubin, hemoglobin and ammonia interfere with biuret assay of total proteins?
Q. I can't find the answers in the internet. They just keep on saying that those interfere with the assay. But they don't specifically tell how each of those interfere with the assay. I need your help badly. Thanks!
Asked by hermione - Tue Sep 2 07:42:40 2008 - - 1 Answers - 0 Comments

A. Read that article
Answered by adel a - Sat Sep 6 07:55:18 2008

You are going to do an enzyme assay, and you need to make a solution of substrate.?
Q. The concentration of substrate that you will use is 0.001 ug/ml. It is necessary to have enough for the enzyme assay. You will need to test 50 samples, plus a negative control lacking the enzyme, and another negative control lacking the substrate. You have determined that you will do all of this in triplicate. The assay calls for 1 ml of substrate solution and 0.2 ml of enzyme solution. If you don't have enough substrate solution, you will have to make more in the middle of the experiment, which could ruin your results. a. How much substrate solution should you make? b. How many grams of substrate will you need for this solution? c. How can you make this solution if you don't have a balance accurate enough to weigh such a small quantity? I… [cont.]
Asked by Lena - Fri Feb 23 07:16:10 2007 - - 1 Answers - 0 Comments

A. a. How much substrate solution should you make? 50 samples + a negative control (do all of this in triplicate) The assay calls for 1 ml of substrate solution Therefore, (50 + 1)x1 ml x 3 = 153 ml b. How many grams of substrate will you need for this solution? concentration of substrate that you will use is 0.001 ug/ml Therefore, 153 ml x 0.001 ug/ml = 0.153 ug c. How can you make this solution if you don't have a balance accurate enough to weigh such a small quantity? Weigh 1.0mg of substrate and dissolve it in 1000 ml buffer solution in a volumetric flask (stock solution). The resulting concentration will be 1.0 mg/1000ml = 0.001mg/mL (i.e. 1 ug/ml ) Take 1ml from stock solution and dilute it to 1000 ml with buffer solution in a… [cont.]
Answered by Yy - Fri Feb 23 10:03:34 2007

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