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An assay is a procedure in molecular biology for testing or measuring the activity of a drug or biochemical in an organism or organic sample. A quantitative assay may also measure the amount of a substance in a sample. Bioassays and immunoassays are among the many varieties of specialized biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding. Assay varietiesThis section does not cite any references or sources. Please help improve this article by adding citations to reliable sources. Unsourced material may be and removed. (May 2009)CytotoxicityA cytotoxicity assay measures how toxic a chemical compound is to cells.
DNAAssays for studying interactions of proteins with DNA include: From Wikipedia under the
GNU Free Documentation License How can the use of detergents in a protein assay effect the protein concentrations? Q. Detergent solution used in this protein assay to make up samples containing a known concentration of BSA, aswell as detergent. Asked by Issy - Sat Feb 28 19:49:42 2009 - - 1 Answers - 0 Comments A. The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method to be used on detergent treated membrane preparations. Answered by unknown - Sun Mar 1 07:24:07 2009 Why does wash buffer is being added together with monoclonal antibody when doing ELISA assay? Q. As i thought that wash buffer is used later on after the monoclonal antibody is added. Would be possible if it helps in diluting the antibody? Asked by b3th - Fri Oct 23 21:25:08 2009 - - 1 Answers - 0 Comments A. Antibodies are active only within a specific pH range. The buffer ensures that the required pH will be maintained. Answered by PaulCyp - Fri Oct 23 23:04:28 2009 How can I test the assay of a Grignard reagent in a reaction mass?
Q. I am preparing a grignard reagent from n-Amyl Bromide Asked by Anil K - Wed Jun 11 06:31:48 2008 - - 1 Answers - 0 Comments A. R-MgBr + H2O --> R-H + MgBrOH On treating an aliquot of the Grignard assay with access of water the reaction mixture turns alkaline. MgBrOH can be titrated whith acid HX to form MgBrX and H2O and the concentration of the Grignard salt can be calculated. Answered by picus48 - Fri Jun 13 14:13:26 2008 From Yahoo Answer Search: "Assay"  marketwire.com Wed, 16 Jun 2010 16:44:35 GMT+00:00 Marketwire (press release) "This is the first time a molecular assay is being used as a primary screening test in newborn screening," says Dr. Baker, who is also an assistant ...  tradingmarkets.com Sun, 20 Jun 2010 12:07:50 GMT+00:00 Trading Markets (press release) The firm is developing a rapid analytical platform for infectious pathogens, the BACcel system, based on its surface coatings, assay processing, ...  7thspace.com Mon, 07 Jun 2010 13:09:23 GMT+00:00 ... 7thSpace Interactive (press release) Several HCV infections can not be detected using currently available commercial assays , probably because of mismatches between the template and ... From Google News Search: "Assay"  aopen.jp 765px x 750px | 138.70kB [source page] http kakaku com item 05500812453 http aopen jp extdowncenter Vga image fx5200dvp128lp assay jpg AH3450+ From Yahoo Image Search: "Assay"  digitalcommons.unl.edu Heather J. Sullivan Mon, 26 Jul 2010 16:36:05 GM An epitope-blocking enzyme-linked immunosorbent . assay. (bELISA) was developed for the detection of antibodies to influenza A virus in taxonomically diverse domestic and wild vertebrate species. In contrast to the bELISAs published ... From Google Blog Search: "Assay" |
